No.  Ultrasound only ever works by line of sight. It will not go through walls or other solid objects.

For best results position the unit in the room where the problem is ensuring the unit has a clear view of the room not being blocked by any objects.

Alternatively, try using the Pest-Stop 2000, which uses an Electromagnetic pulse, carried by your home's internal cabling, to provide coverage up to an entire 3-bed house.